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Removal of Polyethylene Glycol from Proteins by Salt‐Induced Phase Separation
Author(s) -
Busby Thomas F.,
Inghmn Kenneth C.
Publication year - 1980
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1980.tb01843.x
Subject(s) - polyethylene glycol , peg ratio , ethylene glycol , salt (chemistry) , chemistry , chromatography , phase (matter) , aqueous solution , biochemistry , organic chemistry , finance , economics
. The use of poly(ethylene glycol) in the purification of plasma and cellular protein is somewhat complicated by the difficulty of removing it from the protein product. The method presented here, quickly and efficiently removes over 95% of the PEG by the simple addition of salts to induce an aqueous two‐phase separation with the PEG in the upper phase and greater than 90% of the protein in the lower phase.