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Application of Microtiter Solid‐Phase Radioimmunoassay to the Determination of Hepatitis Be Antigen
Author(s) -
Miyakawa Y.,
Tsuda F.,
Akahane Y.,
Mayumi M.
Publication year - 1979
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1979.tb04439.x
Subject(s) - radioimmunoassay , antigen , immunodiffusion , microtiter plate , chromatography , ouchterlony double immunodiffusion , chemistry , hepatitis b antigens , antibody , immunoradiometric assay , microbiology and biotechnology , virology , immunology , hepatitis b virus , biology , antiserum , biochemistry , virus
. Microtiter solid‐phase radioimmunoassay (micro‐SPRIA) was applied to the detection of hepatitis Be antigen (HB e Ag) in the serum. Antibody against HB e Ag to coat the microtiter plate and for radiolabeling was obtained from the serum of asymptomatic carriers of hepatitis B surface antigen by an affinity column of partially purified HB e Ag. The micro‐SPRIA was sensitive and could be performed without prior concentration of the test serum. HB e Ag was detected in 21 (35%) out of 60 serum samples positive for hepatitis B surface antigen, all of which were negative by the conventional immunodiffusion method even after they had been concentrated threefold.