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Characteristics of Different Rhesus Alloantibodies in Lymphocyte Dependent (K Cell) Cytotoxicity to Human Erythrocytes
Author(s) -
Lajos J.,
Benczur M.,
Nemák P.
Publication year - 1979
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1979.tb04431.x
Subject(s) - antibody dependent cell mediated cytotoxicity , cytotoxicity , antibody , antigen , immunology , microbiology and biotechnology , lymphocyte , in vitro , isoantibodies , biology , autoantibody , chemistry , monoclonal antibody , biochemistry
. The property of rhesus alloantibodies to elicit antibody‐dependent, cell‐mediated cytotoxicity (ADCC) against target erythrocytes carrying various Rh genotypes was studied. The killer activity of normal peripheral lymphocytes on human erythrocyte target cells carrying the appropriate antigens elicited by alloantisera was measured by 51 Cr release at 18 h. There was no correlation between ADCC and antibodies directed to the antigens present on the surface of different genotypes of Rh‐positive red blood cells. The agglutinin titre of different Rh antibodies showed no correlation with the level of ADCC although the degree of cellular cytotoxicity was different with different anti‐D sera. Anti‐C + D + E antibody caused higher ADCC than anti‐C + D and the lowest cytotoxicity was observed with anti‐D and anti‐D + E. This raised the possibility that ADCC was elicited by antibodies directed to other specificities. K cell lysis of human red cells by human peripheral blood lymphocytes in vitro suggests that a similar mechanism may operate in vitro in the destruction of erythrocytes coated by alloor autoantibodies.