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An Electronic Method for Counting Human T and B Cell Rosettes 1
Author(s) -
Denny Thomas N.,
Kinsley Kim M.,
Benson Jack W.,
Bull Robert W.
Publication year - 1979
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1979.tb02298.x
Subject(s) - hemocytometer , enumeration , cell counting , rosette (schizont appearance) , mathematics , cell , chemistry , immunology , biology , combinatorics , genetics , biochemistry , cell cycle
. This study reports the adaptation of an electronic particle‐counting method for the enumeration of T cell and B cell rosettes of human lymphocytes. This method and the conventional hemocytometer method were compared in parallel on seventeen T cell and seven B cell rosette enumerations in a blind study design. All data were analyzed for statistical significance; correlation coefficients were calculated. There was significant agreement between both methods for Tcells at p<0.001 (mean particle counter 80.6% ±11.3, mean hemocytometer 76.7 ± 8.2%) and for the B cells at p<0.002 (mean particle counter 22.5±9.7, mean hemocytometer 23.4±5.12). These results demonstrate that the electronic particle counter is useful as a rapid, convenient, semi‐automated method for the enumeration of T cell and B cell rosettes.