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A New Case of Gamma Heavy Chain Disease: Clinical, Immunochemical and Structural Characterization
Author(s) -
Carbonara A.O.,
Paolino W.,
Malavasi F.,
Levis A.,
Stramigi A.,
Lange G.,
Loghem E.,
Franklin E. C.
Publication year - 1979
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1979.tb02276.x
Subject(s) - gamma globulin , dimer , subclass , antigen , polyacrylamide gel electrophoresis , immunoelectrophoresis , electrophoresis , chemistry , residue (chemistry) , microbiology and biotechnology , antibody , biology , biochemistry , immunology , enzyme , organic chemistry
. A new case of gamma heavy chain disease (γHCD) is described in a 77‐year‐old woman. The serum and urine contain an M‐component with electrophoretic, antigenic and ultracentrifugal properties resembling those of the Fc fragment of IgG globulin. Analysis on SDS‐polyacrylamide gel electrophoresis and ultracentrifugal studies show that γHCD protein is present in the serum as a dimer with a molecular weight of 58,000 daltons. Analysis of isotypic and allotypic markers along with the structural studies show that this HCD protein belongs to the IgGl subclass and that deletion includes the total V H and C H1 regions with sequence starting at residue 225 in the middle of the hinge region.