Premium
An Improved Method for the Identification of Gc 1 Subtypes (Group‐Specific Component) by Isoelectric Focusing
Author(s) -
Kühnl P.,
Spielmann W.,
Loa M.
Publication year - 1978
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1978.tb02955.x
Subject(s) - isoelectric focusing , allele , genetics , biology , gene , microbiology and biotechnology , population , allele frequency , german population , medicine , biochemistry , environmental health , enzyme
. The occurrence of common subtypes of the Gc 1 gene in a German population (n = 261) was investigated by isoelectric focusing on thin layers of polyacrylamide in the pH range 4–6. Six common phenotypes designated Gc IS, IF, 1F‐1S, 2‐1S, 2‐1F, and 2 are considered as gene products of three common alleles with the following frequencies: Gc 1S = 0.603, Gc 1F = 0.125, and Cc 2 = 0.272. The sum of the allele frequencies of Gc 1S and Gc 1F corresponds with that of the ‘old’ Gc 1 allele. Family investigations are in agreement with an autosomal codominant way of inheritance. The method employed provides an identification of the subtypes within 4 h without using anti‐Gc‐serum for immunoprecipitation.