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Maintenance of 2, 3‐DPG and ATP Levels in Blood Stored for 30 Days at a Constant pH Obtained by Variation of CO 2
Author(s) -
Mendonca M.,
Appel M.,
Bidault J.,
MassonPierre D.,
Terrier E.
Publication year - 1978
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1978.tb02920.x
Subject(s) - variation (astronomy) , constant (computer programming) , blood preservation , chemistry , zoology , medicine , andrology , biology , physics , computer science , astrophysics , programming language
. A novel procedure for storing blood at a controlled pH consists in collecting blood in a pH 8.20, Tris‐CPD solution and storing it in a special recipient including a gas permeable membrane under a CO 2 atmosphere. The recipient is placed in an atmosphere of variable CO 2 content, so that the initial alkalinity of the preservative is balanced by dissolved CO 2 , the proportion of which is diminished when lactate production increases with storage. 2, 3‐DPG and ATP were studied at three different pH levels of approximately 7.25, 7.45, and 7.65 at 4°C. The best pH for the simultaneous maintenance of 2, 3‐DPG and ATP was 7.65. Under these conditions, 2, 3‐DPG is maintained at its initial level and ATP at 55% of its initial level at the 30th day. Lactate production is linear and hemolysis moderate.