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Anti‐D Immunoglobulin Preparations: the Stability of Anti‐D Concentrations and the Error of the Assay of Anti‐D
Author(s) -
HughesJones N. C.,
Hunt Valerie A.,
Maycock W. d'A.,
Wesley E. D.,
Vallet L.
Publication year - 1978
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1978.tb02906.x
Subject(s) - confidence interval , antibody , value (mathematics) , chromatography , chemistry , immunology , medicine , mathematics , statistics
. An analysis of the assay of 28 preparations of anti‐D immunoglobulin using a radioisotope method carried out at 6‐monthly intervals for 2–4.5 years showed an average fall in anti‐D concentration ot 10.6% each year, with 99% confidence limits ot 6.8–14.7%. The fall in anti‐D concentration after storage at 37°C for 1 month was less than 8%, the minimum change that could be detected. No significant change in physical characteristics of the immunoglobulin were detected. The error of a single estimate of anti‐D by the radioisotope method ( 125 I‐labelled anti‐IgG) used here was calculated to be such that the true value probably (p = 0.95) lay between 66 and 150% of the estimated value.