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Variable Degradation of Factor VIII‐Related Protein in Lyophilised Concentrates of Antihaemophilic Factor (AHF) 1
Author(s) -
Jakab T.,
Pflugshaupt R.,
Furlan M.,
Beck E. A.
Publication year - 1978
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1978.tb02898.x
Subject(s) - degradation (telecommunications) , chemistry , chromatography , medicine , computer science , telecommunications
. Factor VIII‐related properties (coagulant = VIII: C, ‘Willebrand’ factor = VIII R: WF, antigen = VIII R: AG) are measured in a constant proportion in normal plasma and certain preparations of highly purified factor VIII (relative ratios: 0.5‐1.5). We tested these activities in some commercial, lyophilised concentrates of factor VIII and found a variable increase of the ratio VIII R: AG/V1II R: WF. The relative increase of VIII R: AG, and/or loss of VIII R: WF, was attributed to variable degradation of factor VIII‐related protein(s) which was directly visualised by electrophoresis on 2.75% polyacrylamide gels in the presence of sodium dodecyl sulphate.