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Comparison of Low‐Molecular‐Weight Products Following Reaction of C3‐C3b with C3b Inactivator and with Trypsin 1
Author(s) -
Freedman J.,
Chaplin H.,
Johnson C.A.,
HughesJones N. C.
Publication year - 1977
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1977.tb04466.x
Subject(s) - trypsin , chemistry , chromatography , biochemistry , enzyme
. Substitution of trypsin for Konglutinogen‐activating factor (KAF) in the procedure for cleaving C3d from C3‐C3b substrate produced a relatively heterogeneous low‐molecular‐weight fraction (C3d‐Tryp) which differed in a number of ways from the KAF‐mediated cleavage product (C3d‐KAF). The differences were demonstrable by agar and polyacrylamide gel electrophoresis, 125 I‐labelling, content of immunoreactive 125 I‐labelled C3d, inhibition of anti‐complement antiglobulin reagents and rabbit immunization. By comparison with C3d‐KAF, the C3d in C3d‐Tryp was more heterogeneous and exhibited a faster electrophoretic mobility in agar at pH 8.6. By contrast to C3d‐KAF, C3d‐Tryp contained protein carrying C3c antigenic determinants.