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Radioimmunoassay of Hepatitis B Core Antigen and Antibody with Autologous Reagents 1
Author(s) -
Vyas Girish N.,
Roberts Isabel M.
Publication year - 1977
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1977.tb02259.x
Subject(s) - radioimmunoassay , antigen , antibody , virology , serology , neutralization , microbiology and biotechnology , immunology , chemistry , medicine , biology , biochemistry
. Anti‐HBc is the most sensitive indicator of current or recent hepatitis B virus infection, hence its detection is valuable in laboratory diagnosis of the infection. Dane particles isolated from the plasma of a single chronic carrier and his autologous IgG labeled with 125 I were utilized in a solid‐phase sandwich radioimmunoassay for anti‐HBc. The principle of serologic neutralization of core antigen and consequent inhibition of binding of the cores to the solid‐phase rendered the method to be a simple, sensitive, specific and rapid means of detecting anti‐HBc.