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HLA‐Antibody‐Induced 14 C‐Serotonin Release from Platelets:
Author(s) -
Heinrich D.,
Gutschank S.,
MuellerEckhurdt C.
Publication year - 1977
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1977.tb02236.x
Subject(s) - platelet , serotonin , antiserum , incubation , chemistry , antibody , immunology , endocrinology , microbiology and biotechnology , medicine , biochemistry , biology , receptor
. The prerequisites and kinetics of HLA‐antibody‐induced 14 C‐serotonin release from platelets were investigated with five HLA‐specific antisera. By studying the influence of incubation time, temperature, pH, platelet count, stirring and extent of platelet labeling, optimal results were obtained after 30 (to 60) min of incubation at 37°C, pH 7.5–8.0, under constant stirring (400 rpm). Optimal platelet counts varied between 160,000 and 400,000/μl depending on the antiserum applied. A comparison of the amount of 14 C‐serotonin released by different antisera from washed platelets and from platelets in platelet‐rich plasma suggests that plasma components ‐ most likely complement ‐ may additionally influence the results. Based on these experiments, a standardized microtechnique of HLA‐antibody‐induced 14 C‐serotonin release is recommended.