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Radioimmunoassay Inhibition Method for Confirming the Specificity of Positive Hepatitis B Surface Antigen Reactions and for Survey of Antibodies to the Antigen 1
Author(s) -
Forghani Bagher,
Schmidt Nathalie J.,
Lennette Edwin H.
Publication year - 1977
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1977.tb00616.x
Subject(s) - radioimmunoassay , hbsag , antigen , antibody , antiserum , titer , heterologous , virology , hepatitis b , microbiology and biotechnology , chemistry , biology , immunology , hepatitis b virus , virus , biochemistry , gene
. Studies were conducted to determine the hepatitis B surface antigen antibody (anti‐HB S ) levels and antibody subtype specificities required in order for anti‐HB S sera of human origin to be suitable for use in radioimmunoassay (RIA) inhibition tests for determining the specificity of positive reactions for hepatitis B surface antigen (HB S Ag.) Also, the RIA inhibition method was evaluated for detection of anti‐HB S in sera of laboratory personnel. Only anti‐HB S sera with complement‐fixing (CF) antibody titers of 1:32 or higher effectively inhibited HB S Ag‐positive sera with demonstrable CF activity, and complete inhibition was obtained only for antigens having CF titers of 1:64 or lower. Higher‐titered antigens could be inhibited when diluted 1:10‐1: 100. The low‐titered HB S Ag preparation supplied with AusRIA kits (Abbott Laboratories) was effectively inhibited by low‐titered anti‐HB S sera, and when used in RIA inhibition tests on sera from laboratory personnel, the tests detected anti‐HB S in approximately 14% of the individuals tested, a slightly greater number than those showing anti‐HB s in passive hemagglutination tests. Inhibition of HB S Ag‐positive sera by the anti‐HB S sera could not be related to the d and y subtype specificities of the antigens or antisera; the antisera were no more effective in inhibiting antigens of the homologous subtype than those of the heterologous subtype.