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Detection of an Antigen in Albumin Subjected to Repeated Heating
Author(s) -
Cohen Pinya,
Roelands Joan F.
Publication year - 1976
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1976.tb04656.x
Subject(s) - albumin , antiserum , chromatography , globulin , chemistry , antigen , serum albumin , blood proteins , microbiology and biotechnology , biochemistry , immunology , biology
Abstract. Stabilized S and 25% normal serum albumin (human) derived from plasma, placentas and plasma‐placental blends was subjected to repeated heating at 56°C for 120 h, interspersed with storage at 4°C for 48 h. Immunoelectrophoretic analyses showed that after the ninth heating, 5% plasma albumin developed a component which migrated in the α‐globulin region and gave a reaction of nonidentity with albumin. This antigen, which was not detected in the other albumin samples, reacted with antiserum prepared against control 5% plasma albumin, indicating that it was present in the latter at an undetectably low concentration.