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Electron Microscopic Observations on the H Antigen Sites of Human Erythrocytes Using Ferritin Antibody Conjugates
Author(s) -
Matsukura Yutaka
Publication year - 1976
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1976.tb04655.x
Subject(s) - abo blood group system , antigen , immunoelectron microscopy , ferritin , microbiology and biotechnology , fetus , antibody , h antigen , isoantigens , chemistry , red blood cell , biology , andrology , immunology , medicine , pregnancy , biochemistry , genetics
. Using immunoelectron microscopy, the distribution of the H antigen sites on human erythrocytes was observed in 40 samples of adult, newborn and fetal blood of different ABO phenotypes. The attached ferritin particles indicating the H antigen sites conspicuously varied in number from cell to cell in every specimen. The number of H antigen sites per single red cell was estimated on an average for each sample as follows: 0, 3 × 10 5 ; B, 2 × 10 5 ; A 1 , 1.5 × 10 5 ; A 1 B, 10 5 ; A 2 B, 1.5 × 10 5 ; Ax, 2.5 × 10 6 ; A ×B, 10 5 ; Bm, 4 × 10 5 ; Bw(leukemia), 4 × lo 5 ; O(newborn), 2.5 × 10 5 ; B(newborn), 3 × 10 5 ; A 1 (new‐born), 1.5 × 10 5 ; A 1 B(newborn), 2 × 10 5 ; A 1 B(fetus), 10 5 . The cells in each sample were divided into six cell‐populations according to the number of H antigen sites present. The ratios of distribution of such cell populations are compared for all samples.