Premium
Application of a High Sensitivity Aggregate‐Haemagglutination Test for the Diagnosis of Autoimmune Haemolytic Anaemia with a Negative Direct Antiglobulin Test
Author(s) -
Idelson L. I.,
Koyfman M. M.,
Gorina L. G.,
Olovnikov A. M.
Publication year - 1976
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1976.tb04454.x
Subject(s) - coombs test , autoantibody , hemagglutination , immunology , medicine , antibody , autoimmune hemolytic anemia
. An aggregate‐haemagglutination test has been used for determining antierythrocyte autoantibodies. The first antiglobulin variant of the test allows us to establish the presence of antibodies in 33 cases with a negative direct Coombs' test. The test II (antiantiglobulin variant) proved to be positive in 88 cases of autoimmune haemolytic anaemia (AIHA) that showed a negative direct Coombs' test and a negative antiglobulin variant. Immunoglobulin G has been revealed in the majority of AIHA patients. IgM has been determined in 1 case of a symptomatic form associated with chronic lymphocytic leukaemia. IgA has been recorded in 3 cases with an idiopathic AIHA form and in 6 cases of chronic lymphocytic leukaemia. Both types of light chains were found on the surface of erythrocytes in all cases of AIHA.