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Radioelectroimmunoassay of Anti‐D Immunoglobulin on Agarose Plates Containing Whole Red Cells
Author(s) -
ERIKSSON OLLE,
ENELL BIRGITTA
Publication year - 1976
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1976.tb02854.x
Subject(s) - agarose , antibody , microbiology and biotechnology , chemistry , immunology , medicine , chromatography , biology
. By incorporating whole Rh(+) red cells in an agarose plate and adding a small amount of purified 125 I anti‐D immunoglobulin as a tracer to standards and samples, a radioelectroimmunoassay (REIA) of anti‐D immunoglobulin was possible. The results by this method agree with those obtained by the direct method. The coefficient of variation (within plate precision) is less than 9% and the reproducibility is 12% (coefficient of variation for a plasma sample assayed on six occasions during 1 month). The International anti‐D serum was compared with the WHO standard preparation 68/419 on five occasions and found to contain 11; 11; 10; 9 and 11 μg per ampoule.