Premium
Purification of Hepatitis B Surface Antigen Using Polyethylene Glycol, Pepsin and Tween 80 1
Author(s) -
Nath Nrapendra,
Mazzur Scorn,
Ledman Robert,
Fang Chyang T.
Publication year - 1976
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1976.tb02226.x
Subject(s) - pepsin , polyethylene glycol , antigen , chemistry , chromatography , peg ratio , antibody , digestion (alchemy) , biochemistry , microbiology and biotechnology , enzyme , immunology , biology , finance , economics
. A simple, inexpensive procedure for the isolation and purification of HB s Ag from plasma is described. The technique included precipitation of HB s Ag with PEG and elimination of normal plasma proteins by digestion with pepsin. Tween 80 was used to remove contaminating lipoprotein(s). This technique resulted in about a 200‐fold gain in the specific activity of HB s Ag and yielded about 20–40% recovery. Rabbits immunized with the purified antigen produced type‐specific antibodies to HB s Ag without detectable reactivity to normal human plasma antigens.