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Quantitative Rh Typing of r G r G with Observations on the Nature of G (Rh12) and Anti‐G
Author(s) -
Rosenfield Richard E.,
Levine Philip,
Heller Charlene
Publication year - 1975
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1975.tb02770.x
Subject(s) - microbiology and biotechnology , rh blood group system , antiserum , antigen , biology , stereochemistry , chemistry , genetics , antibody
. Quantitative blood typing data on Mr. R. B. (r G r G ), his wife (R 1 r), a daughter (R 1 r G ), and a niece (r G r) strongly suggest that these Rh phenotypes are directly indicative of actual Rh genotypes. If so, the antigenic products of r G are weak Rh2 (rh' or C), normal Rh5 (hr or e), an expression of Rh12 (rh G ) equal to that produced by R ‐1,2,‐3 ( r ' or dCe ) and half of what is produced by R 1 ( R or D ) genes in either homozygous or heterozygous expression, very weak expression of Rh19 (hr S ), absence of Rh31 (hr B ), and slightly weakened expression of Rh17 (Hr o or ‘not D’). The LW status of r G r G cells was equivalent to that of other Rh:‐1 erythrocytes. Thus r G resembles mutant r' in which only Rh5 is expressed normally. The weak Rh2 produced by r G reacted much better with one Rh2 antiserum than with another. Rh21 (C G ) had been used to denote such additional reactivity, but one reagent that acted as anti‐Rh2 in manual tests behaved like anti‐Rh21 in instrumented tests. Therefore, anti‐Rh21 may only indicate a more efficiently agglutinating anti‐Rh2. Mr. R. B. showed no evidence of congenital stomatocytic hemolytic anemia characteristic of Rh null or Rh mod. Finally, anti‐Rh12 eluates, recovered either sequentially from r'r followed by R 2 r or singly from r G r G , agglutinated chimpanzee red cells more efficiently than did either anti‐Rh1 (D) or anti‐Rh4 (c), a result consistent with expectation for serological crossreactivity between Rh1 and Rh21.