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Plasmapheresis: the in vitro Distribution of Heparin in Plasma and Red Cell Fractions following Centrifugation
Author(s) -
Mishler John Milton
Publication year - 1975
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1975.tb02760.x
Subject(s) - plasmapheresis , heparin , centrifugation , partial thromboplastin time , whole blood , chemistry , in vivo , chromatography , in vitro , red blood cell , blood cell , red cell , pharmacology , immunology , medicine , biochemistry , biology , platelet , antibody , microbiology and biotechnology
. A modified activated partial thromboplastin time (activated PTT) technique was employed in determining heparin concentration in plasma following centrifugation of whole blood. In reference to previous studies reporting heparin to be found essentially in the plasma, it was shown in the present study that following centrifugation of each bag of whole blood in a double‐pheresis procedure, the approximate heparin distribution was 39.9 and 45.7% recovery in plasma for bags 1 and 2, respectively. It is suggested that heparin binding to cellular components may account for the low recovery in plasma. Activated PTTs conducted following reinfusion of red cell concentrates into donors were within normal values, and were consistent with the suggestion that heparin is bound to cellular components in vivo. The safety of the weekly injection of approximately 3,120 USP units of heparin into donors on a long‐term basis is considered in the light of extensive data avilable from widespread systemic use.