Premium
A Simple Method for the Prediction of ABO Incompatibility Using Sephadex A‐50:
Author(s) -
Graham H.,
Morrison M.,
MacAndrew R.
Publication year - 1975
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1975.tb00521.x
Subject(s) - abo blood group system , titer , sephadex , chromatography , titration , chemistry , immunology , medicine , antibody , biochemistry , inorganic chemistry , enzyme
. A rapid and simple method of demonstrating the presence of high titre IgG anti‐A or anti‐B (anti A/B) is described. The method requires the separation of IgG anti‐A/B from IgM anti‐A/B using DEAE Sephadex A‐50 by a simple spin technique, and the subsequent titration of the IgG fraction to detect the presence of high titre IgG anti‐A/B. Since high titre IgG anti‐A/B is usually associated with ABO incompatibility the test is a useful guide to the prediction of ABO incompatibility in routine antenatal samples.