Premium
Platelet Indirect Radioactive Coombs Test. Its Utilization for Pla 1 Grouping
Author(s) -
Soulier J. P.,
Patereau Claude,
Drouet J.
Publication year - 1975
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1975.tb00506.x
Subject(s) - platelet , complement fixation test , coombs test , immunology , antibody , chemistry , medicine , serology
. A platelet indirect radioactive Coombs test (PIRC) has been described. The technique for purification and labelling the antiglobulin has been precised. This test allowed the typing of platelets in the PLA system by using an absorbed serum from a mother of a thrombocytopenic child. Six other families of neonatal thrombocytopenias were tested. In three of them, the mothers were found PLA 1 negative (PLA 2 , PLA 2 ). Among a panel of 93 platelets, two (0.022) were found PLA 1 , negative. This PIRC test has many advantages compared to other tests such as platelet complement fixation, assay for blocking antibodies or antiglobulin consumption: it gives objective and quantitative results and is highly reproducible; anticomplementary serum may be tested.