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Blood‐Group‐Like Substances in Some Marine Invertebrates
Author(s) -
Renwrantz Lothar,
Uhlenbruck Gerhard
Publication year - 1974
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1974.tb02710.x
Subject(s) - ouchterlony double immunodiffusion , chemistry , biochemistry , degree of unsaturation , glycoprotein , agglutinin , anomer , galactosamine , stereochemistry , biology , glucosamine , lectin , chromatography , immunology , antigen , antiserum
. Blood group A reactive substances have been proved to occur in extracts of Amphioxus (Branchiostoma) lanceolatus and in the serum of Phallusia mammilata. Using the Ouchterlony test, the A‐like structures could be precipitated with anti‐A HP , which reacts with the α‐ and β‐anomer of terminal, nonreducing bound N ‐acetyl‐D‐galactosamine. The precipitation test with the agglutinin of Cepaea hortensis (anti‐A HH ), which has an affinity only for the respective α‐anomer, was positive, too. As both results could be reobtained after a lipid extraction by treatment with phenol, the conclusion can be drawn that the tested ascidian and the lancelet contained blood group A reactive glycoproteins with terminal, nonreducing bound α‐ N ‐acetyl‐D‐galactosamine.