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Immunodiffusion Studies of Blood Group A Antigen 1
Author(s) -
Milgrom F.,
Mohn J. F.,
Loza Ulana
Publication year - 1974
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1974.tb02680.x
Subject(s) - population , antiserum , saliva , antigen , lectin , immunodiffusion , group a , antibody , biology , immunology , medicine , biochemistry , environmental health
. Stromata of blood group A 1 erythrocytes fragmented by ultrasound were studied by means of double diffusion in gel. In many instances the reactions with immune anti‐A sera were composed of two lines. Evidence was presented that group A 1 secretor saliva contains all the A antigenic specificities present on the stromata. On the other hand, group A 1 secretor serum appeared to be antigenically poorer than stromata. The reaction line with group A 1 stromata was stronger than with group A 2 stromata. Some evidence for a qualitative antigenic difference between the group A 1 and A 2 stromata was also obtained. Phaseolus limensis lectin produced a reaction line with group A 1 stromata. Data were presented in support of the existence of two populations of stromal particles, one combining with this lectin and anti‐A serum and the other with the antiserum only. The first population of particles could be eliminated from the stromal suspension without influencing the reactivity of the sedond population.