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The Electrophoretic Properties of Some Human Blood Cells
Author(s) -
Zerial A.,
Welkins David J.
Publication year - 1974
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1974.tb02661.x
Subject(s) - electrophoresis , ionic strength , chemistry , globulin , lymphocyte , ionic bonding , surface charge , biophysics , immunology , microbiology and biotechnology , biochemistry , biology , ion , organic chemistry , aqueous solution
. Human red cells, granulocytes and lymphocytes were examined electro‐phoretically. The effect of change of pH and ionic strength of the medium was determined. Lowering the ionic strength had the effect, not only of altering the electrophoretic mobility but also the characteristic shape of the pH mobility curves. Antilymphocyte globulin was shown to modify the mobility of granulocytes and lymphocytes, the latter to a greater extent. The results when compared to a silica surface completely covered with horse antihuman lymphocyte globulin show that the lymphocyte is only partially covered, suggesting a relatively low number of antigenic sites on the surface.