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Biochemical Changes on Storage of Blood
Author(s) -
Ioppolo Carmela,
Amiconi Gino,
Currell Douglas L.,
Maffei Gennaro,
Zolla Lello,
Antonini Eraldo
Publication year - 1974
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1974.tb02437.x
Subject(s) - hemoglobin , chemistry , ageing , oxygen , tris , red cell , red blood cell , biochemistry , phosphate buffered saline , biophysics , chromatography , biology , medicine , organic chemistry
. The rate of reduction of ferrihemoglobin within the erythrocytes and the oxygen affinity of red cells have been measured as a function of time of blood storage. The efficiency of the ferrihemoglobin‐reducing systems decreases with time of storage as measured both in presence of glucose or lactate as substrates. The well‐known increase with ageing of the oxygen affinity (as measured by p1/2) for blood stored in ACD is observed when the p1/2 is determined in Tris‐HCl buffer and parallels the decay of the rate of ferrihemoglobin reduction. However, the increase in oxygen affinity is not observed when the p1/2 is measured in phosphate buffer; this is explained as being due to the replacement of the originally hemoglobin‐bound DPG by phosphate ions entering the red cell.