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Separation of Hepatitis‐Associated Antigen (HAA) from Human Plasma for the Production of Rabbit Anti‐HAA
Author(s) -
Booth J. R.,
Wiseman I. C.,
Lee D.
Publication year - 1974
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1974.tb02412.x
Subject(s) - chemistry , chromatography , ouchterlony double immunodiffusion , sephadex , antigen , reagent , elution , immunodiffusion , size exclusion chromatography , electrophoresis , biochemistry , immunology , biology , antiserum , enzyme
. A method is described for obtaining hepatitis‐associated antigen (HAA) from the acid citrate dextrose plasma of routine blood donations found to be HAA‐positive. The separation consisted of the following steps: ammonium sulphate precipitation, absorption on and elution from DEAE‐Sephadex, and gel filtration on a Sepharose‐6B column. HAA was demonstrated by cross‐over immuno‐electrophoresis and lines of identity shown by the Ouchterlony double diffusion technique. Freedom from contaminating protein was shown by immuno‐electrophoresis. This material, when injected into rabbits, produced an anti‐HAA reagent suitable for use cross‐over immuno‐electrophoresis without absorption.