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Contributions to the Optimal Use of Human Blood
Author(s) -
Vogelaar E. F.,
Berg M. A. G. D. BoerV. D.,
Brummelhuis H. G. J.,
Beentjes S. P.,
Krijnen H. W.
Publication year - 1974
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1974.tb02409.x
Subject(s) - antibody , fractionation , yield (engineering) , chemistry , immunoglobulin g , chromatography , fraction (chemistry) , immunology , biology , materials science , metallurgy
. A quantitative analysis was performed of the method of plasma fractionation used in our institute, a modified Cohn VI system, in order to develop and evaluate methods for the increment of antibody yield and quality of the immunoglobulin concentrate. For this purpose the distribution of the immunoglobulins IgG, IgM, IgA, the subclasses of IgG and several specific antibodies in the different fractions was analysed. In addition, the distribution of some plasma proteins which may be of importance for the quality of the immunoglobulin concentrate, such as α 2 M, α 1 ‐antitrypsin, α 2 ‐lipoprotein and fibrinogen, was determined. The average yield of IgG of immunoglobulin concentrates prepared from 31 plasma batches was 58% (SD 9%). Loss of IgG predominantly occurred in fraction III with an average of 25% (9 batches). Most of the measured antibody activities were similar to that of IgG. The anti‐D antibody showed a lower yield, the mean value of 13 batches was 41 % (SD 11 %), which could be attributed to a higher loss in fractions I and III. The steps in the ethanol fractionation in which considerable losses occurred, were analysed in order to improve the yield of immunoglobulins, especially of anti‐D. Suggestions for modifications leading to improvement of the yield are given.