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The Role of Enzymes and Albumen in Haemagglutination Reactions: A Serological und Ulrrastrucrural Study with Ferritin‐Labelled Anti‐D
Author(s) -
Voak D.,
Cawley J. C.,
Emmines J. P.,
Barker C. R.
Publication year - 1974
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1974.tb02403.x
Subject(s) - neuraminidase , papain , agglutination (biology) , hemagglutination , chemistry , enzyme , protease , antibody , biochemistry , ferritin , microbiology and biotechnology , biology , immunology
. In albumen and papain tests, strong haemagglutination showed large areas of cell to cell contact, whereas neuraminidase only caused loose agglutination with small areas of cell to cell contact, confirming a previous suggestion that reduced zeta‐potential is not the significant factor in Rh haemagglutination. The ferritin‐labelled anti‐D was only clustered on neuraminidase‐ and papain‐treated cells, possibly these enzymes cause limited membrane mobility. It is postulated that the loose agglutination in neuraminidase tests is due to localised high density of D sites and that the much greater agglutinating activity in papain tests is due to clustering and protease activity. Increased anti‐D antibody uptake is not a critical factor in enzyme tests because cells sensitized and then washed before enzyme treatment were strongly agglutinated. Recent studies have revealed that dextran does not reduce zeta‐potential but acts by adsorption between adjacent cells. We suggest that a similar mechanism may explain the action of albumen.