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Leukocyte‐Poor CPD Blood
Author(s) -
FreyWettstein M.,
Bachmann Marlies
Publication year - 1974
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1974.tb02385.x
Subject(s) - buffy coat , centrifugation , red cell , andrology , red blood cell , chromatography , chemistry , immunology , medicine
. A simple method is described for the preparation of leukocyte‐poor red cell concentrates from fresh and stored CPD blood. Warm normal CPD blood is passed through a nylon wool filter. After centrifugation at 1,500 g for 3 min, the plasma, the buffy coat and the top 30 ml of the red cell concentrate are eliminated. The average removal of leukocytes is better than 97%, the relation of remaining lymphocytes and granulocytes being 1:1. Red cell loss ranges around 20%. Advantages and disadvantages of the preparation are discussed.