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Separation of Platelet Rich Plasma and Red Cells with Modified Gelatin
Author(s) -
Kummer H.,
Schwander D.,
Dezaules M.,
Mosimann W.
Publication year - 1973
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1973.tb03859.x
Subject(s) - phlebotomy , platelet , gelatin , platelet rich plasma , chemistry , centrifugation , andrology , adhesion , in vitro , platelet poor plasma , red cell , whole blood , chromatography , medicine , immunology , surgery , biochemistry , organic chemistry
. The l'reparation of platelet rich plasma (PRP) by acceleration of red cell sedimentation with modified gelatin is evaluated. The mean recordings in the supernatants of 25 blood units after a sedimentation time of 92 min are a platelet yield of 88% and less than 1% of red cells in 60% of the phlebotomy volume. The addition of modified gelatin to freshly drawn blood did not alter the platelet function or viability as measured by in vitro aggregation to ADP, adhesion to glass bead columns, bleeding time following a load of 1,500 ml in surgical patients and survival in normal recipients after storage at 22 °C to 72 h. PRP preparation with modified gelatin necessitates a minimal mechanical equipment and may therefore prove particularly valuable for platelet substitution in non‐hospitalized patients.