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The Use of an Immunoadsorbent in the Isolation of Australia Antigen
Author(s) -
Houwen B.,
Marrink J.,
Vos J.,
Molenaar I.,
Nieweg H. O.
Publication year - 1973
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1973.tb03524.x
Subject(s) - immunoadsorption , immunodiffusion , size exclusion chromatography , chromatography , antigen , ultracentrifuge , chemistry , isolation (microbiology) , analytical ultracentrifugation , antibody , filtration (mathematics) , biochemistry , enzyme , biology , microbiology and biotechnology , immunology , statistics , mathematics
. Australia antigen (Au‐ag) was isolated in a four‐stage procedure: pepsin hydrolysis, ultracentrifugation, gel filtration and immunoadsorption. The first three steps serve to remove serum proteins while leaving Au‐ag intact. In the immunoadsorption the remaining contaminant serum proteins are precipitated. A solid phase double antibody system was chosen. The effectiveness of the method was checked in various ways: double immunodiffusion, the production of a hyperimmune serum, gel filtration and immunofluorescence. Morphological integrity was checked by electron microscopy in every stage of the isolation.