Premium
A Therapeutic Concentrate of Coagulation Factors II, IX and X from Citrated, Factor VIII‐Depleted Plasma
Author(s) -
Middleton Sarah M.,
Bennett Ida H.,
Smith J. K.
Publication year - 1973
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1973.tb03485.x
Subject(s) - factor ix , coagulation , chromatography , chemistry , fractionation , heparin , antithrombin , absorption (acoustics) , haemophilia b , haemophilia , haemophilia a , medicine , biochemistry , surgery , materials science , composite material
. A simple procedure is described for the large‐scale absorption on to DEAE‐cellulose of coagulation factors II, IX and X from citrated, factor VIII‐depleted plasma. The coagulation factors are eluted frontally from the exchanger in a high yield and in a form suitable for therapeutic use, without further fractionation. The lyophilised concentrate is very stable without the addition of heparin and, when redissolved to isoosmolar solution, contains approximately 30 U/ml factors II, IX and X, 250–300 times purified from the starting plasma. The effectiveness of the concentrate in the treatment of haemophilia B is discussed.