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Low Protein Rh Immune Globulin (Rh IgG)
Author(s) -
Bowman J. M.,
Chown B.,
Pollock J.
Publication year - 1973
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1973.tb02647.x
Subject(s) - chemistry , autoanalyzer , clearance , immunoelectrophoresis , globulin , ultracentrifuge , antibody , immunology , chromatography , medicine , urology
. After 16–32 months in solution at 4°C, ultracentrifuge and immunoelectrophoresis assays of five lots of low protein Rh IgG protein concentrations 3.9, 5.8, 4.1, 3.4 and 3.1%, revealed each lot to consist of a single unaggregated and unfragmented protein with an s 20 w constant of 6.5–7.3. The lots were 94.0–98.5% pure IgG. Autoanalyzer studies demonstrated no loss of anti‐D for 16–37 months except in one lot stable at 20 months which showed a drop from 363 to 298 μg/ml at 26 months. 125 I antiglobulin measurements revealed a 23‐ to 40‐percent early loss of anti‐D with stability thereafter. Low protein Rh IgG cleared Rh‐positive cells from 11 Rh‐negative volunteers and protected 2,093 Rh‐negative women at risk of Rh immunization at least as effectively as standard Rh IgG. Low protein Rh IgG is a pure, stable, active, protective and economical agent for use in Rh prophylaxis.