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The Effect of Surfactant in the Auto‐Analyser 1
Author(s) -
Gunson H. H.,
Phillips P. K.,
Stratton F.
Publication year - 1972
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1972.tb05124.x
Subject(s) - analyser , pulmonary surfactant , chemistry , chromatography , volume (thermodynamics) , analytical chemistry (journal) , physics , biochemistry , thermodynamics
Summary Experiments have shown that the degree of agglutination of anti‐D and Rh(D) positive cells is reduced as the concentration of the surfactant Tween 20 in the Auto‐Analyser manifold is increased from 0.01 to 0.16%. This leads to a decrease in the sensitivity of the method which may affect antibody detection. It is recommended that the concentration of Tween 20 of 0.02% should not be exceeded and the use of drops to measure the quantity required, a procedure usually recommended, should be replaced by using a measured volume from a stock solution of 10.0%.