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Preparation of Human IgA as By‐Product of Routine Fractionation
Author(s) -
Pejaudier L.,
Audran R.,
Steinbuch M.
Publication year - 1972
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1972.tb03998.x
Subject(s) - chromatography , distilled water , chemistry , fractionation , extraction (chemistry) , fraction (chemistry) , elution , yield (engineering) , materials science , metallurgy
. IgA is prepared from fraction III by a variety of techniques, the most suitable being the extraction of the paste with distilled water, precipitation of the bulk of proteins with caprylic acid, and batch adsorption of IgA on DEAE‐cellulose at pH 5.7 (0.015 m acetate buffer), elution being achieved with 0.09 m acetate buffer. The purified protein contains no IgM, but some IgG (2±1%), the yield being 0.4–0.6 g/100 g paste of fraction III.