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Anti‐β‐Lipoprotein Activity of Human Monoclonal Immunoglobulins
Author(s) -
Riesen W.,
Noseda G.,
Bütler R.
Publication year - 1972
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1972.tb03989.x
Subject(s) - papain , antibody , chemistry , immunodiffusion , monoclonal antibody , lipoprotein , low density lipoprotein , antigen , ouchterlony double immunodiffusion , microbiology and biotechnology , radial immunodiffusion , paraproteins , monoclonal , biochemistry , enzyme , cholesterol , biology , immunology , antiserum
. Human sera containing monoclonal immunoglobulins were screened for antibody‐like activity against lipoproteins by immunodiffusion tests and the passive hemagglutination of low‐density lipoprotein (LDL) coated red cells. Weak positive reactions with human LDL were observed in about 10% of fresh sera. All sera reacted with the patient's own LDL and did not display any specificity against the 8 iso‐antigens of the Ag‐system. No reaction with human high‐density lipoproteins (HDL) and with LDL of animals could be detected. In the case of IgA and IgG myeloma proteins the binding activity was due to the M‐component. The Fab fragment obtained after enzymatic digestion of myeloma IgG with papain could be shown to be the reactive part of the immunoglobulin molecule. The described antibody‐like activity was associated with hypolipidemia.