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Contributions to the Optimal Use of Human Blood
Author(s) -
Vogelaar E. F.,
Brummelhuis H. G. J.,
Beentjes S. P.,
Krijnen H. W.
Publication year - 1972
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1972.tb03842.x
Subject(s) - centrifugation , albumin , chromatography , chemistry , bottle , centrifuge , fractionation , blood proteins , human albumin , fraction (chemistry) , whole blood , biochemistry , surgery , medicine , materials science , physics , composite material , nuclear physics
. Analysis of the production of plasma protein fraction (PPF) showed a considerable loss of albumin (18 to 25% of the amount present in whole blood) in the cell concentrate after centrifugation of blood and in fraction IV (11% of the amount present in plasma) during ethanol fractionation with a modified Cohn VI method. The recovery of albumin could be increased by washing 10 volumes of cell concentrate, derived from blood centrifuged in a ‘bottle centrifuge’ with one volume of 6% NaCl solution. After centrifugation, 120 ml supernatant per unit of blood is obtained with a protein content of 3%. This diluted plasma is mixed with normal plasma and fractionated according to the modified Cohn VI method. The effect of the described modifications is that the quantity of PPF obtained from blood centrifuged in a ‘bottle centrifuge’ is at least 30% more, not only by the recovery of the plasma proteins formerly remaining in the red cell suspension, but also by a decreased loss of albumin in fraction IV. The final product meets the minimum requirements for PPF. When the blood is centrifuged in a continuous centrifuge, the loss of albumin could be decreased from 18 to 5% by using 2.6% trisodiumcitrate and 0.72% NaCl as an anticoagulant solution instead of ACD and by a lower flow rate.