Premium
A Method for Preparing Buffy Coat‐Poor Blood for Transfusion: A Modification
Author(s) -
Goldmann S. F.,
Heiss F.
Publication year - 1971
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1971.tb04813.x
Subject(s) - buffy coat , medicine , platelet , centrifugation , filtration (mathematics) , whole blood , blood transfusion , dextran , transplantation , immunology , surgery , chemistry , chromatography , statistics , mathematics
. Transplant recipients previously sensitized to leukocytes and platelets by pregnancy and transfusion are subject to hyperacute allograft rejection after transplantation. Current methods for preparing buffy coat‐poor blood for transfusion utilize differential centrifugation, filtration of heparinized whole blood, or sedimentation of the red blood cells in the presence of dextran. The average removal of leukocytes with these methods is 70–85%. The procedure described in the present paper increases leukocyte and platelet removal to 96% by passing blood that has been sedimented in the presence of dextran through nylon fibre filters. It is suggested that the technique is an effective method for preparation of buffy coat‐poor blood for prospective allograft recipients.