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The Demonstration of Anti‐HI/HI‐H Activity in Seed Anti‐H Reagents
Author(s) -
Voak D.,
Lodge T.W.
Publication year - 1971
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1971.tb01798.x
Subject(s) - reagent , chemistry , ulex europaeus , agglutinin , chromatography , titration , lotus , nuclear chemistry , biochemistry , botany , biology , organic chemistry , lectin
. Extracts from seeds of Lotus tetragon., Cytisus sessil , and Laburnum alp. give lower titration scores with O H+ ii cells than with O H + I+ cells although both have a similar H status in studies with Ulex europ. reagent. Absorption and elution studies indicate that the Lotus extract contained anti‐HI agglutinins with little activity for O H + ii cells whereas the Cytisus and Laburnum extracts contained anti‐HI‐H agglutinins that cross‐reacted with O H + ii cells. Lactose (7.1 g/100 ml) strongly inhibited the anti‐HI‐H reactions with little effect on the Ulex anti‐H reagent. The anti‐HI/HI‐H reagents gave maximum activity at lower temperatures whereas the Ulex anti‐H extract was just as active at 37°C as at 40°C.