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Observations of the Number of Available c, D, and E Antigen Sites on Red Cells
Author(s) -
HUGHESJONES N. C.,
GARDNER BRIGITTE,
LINCOLN P. J.
Publication year - 1971
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1971.tb00578.x
Subject(s) - antigen , microbiology and biotechnology , antibody , phenotype , chemistry , biology , immunology , biochemistry , gene
Abstract. The number of available antigen sites within the Rh system were estimated using trace‐labelled antibodies. The results were as follows, given as the number of sites per red cell: c‐antigen: on cc cells, 70,000–85,000: on Cc, 37,000–53,000; D sites on cells of phenotype ‐D‐: 110,000–202,000; E‐antigen sites showed considerable heterogeneity depending on phenotype as well as source of anti‐E, and estimates varied between 450 and 25,600; e‐antigen: on eE cells, 13,400 and 14,500: on ee cells, 18,200 and 24,400. The average equilibrium constants of the antibodies were: anti‐E, 4 × 10 8 1/mol; anti‐e, 2.5 × 10 8 1/mol; anti‐c, 3.2 × 10 7 and 5.6 × 10 7 1/mol.