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Australia Antigen Detection by a Rapid Automated Complement Fixation Method
Author(s) -
Sturgeon P.,
Kwak K. S.,
Gitnick G.
Publication year - 1971
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1971.tb00465.x
Subject(s) - autoanalyzer , automated method , hemolysis , complement fixation test , titer , antigen , immunodiffusion , fixation (population genetics) , chromatography , immunology , biology , chemistry , computer science , antibody , artificial intelligence , biochemistry , serology , gene
. An automated complement fixation test using a continuous flow system based on AutoAnalyzer components in which sheep cell hemolysis is detected in an optical cell counter has been developed. With this system 1,680 donor bloods diluted 1/10 were tested in parallel with manual immunodiffusion (ID) and microtiter complement fixation (CF) tests; 16 positive samples were found. The manual and automated CF tests were in agreement in 14 of these but in 2 additional instances, where the automated test was negative, the manual test gave a minimal positive titer of 8. With ID, 9 of the 16 were positive. The automated test results are available in 45 min at a rate of 60/h. The system is mechanically compatible with other automated blood banking systems.