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A Method for the Preservation of Human Blood Group Erythrocyte Antigens in Liquid Nitrogen for a Test Cell Panel 1
Author(s) -
MOHN J. F.,
BOWMAN H. S.,
CUNNINGHAM R. K.
Publication year - 1970
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1970.tb01783.x
Subject(s) - lysis , liquid nitrogen , cryopreservation , sorbitol , glycerol , red blood cell , andrology , blood preservation , intracellular , chemistry , cryoprotectant , blood urea nitrogen , red cell , lytic cycle , antigen , chromatography , electrolyte , immunology , biochemistry , medicine , biology , creatinine , embryo , virus , organic chemistry , microbiology and biotechnology , electrode
. A modification of Krijnen's cryopreservation method, using low intracellular concentrations of 17.5% glycerol and extracellular 4% sorbitol as additives, has been developed for liquid nitrogen freezing of small volumes of erythrocytes for a test cell panel. A technique for their subsequent successful storage in sterile, dextrose‐electrolyte maintenance solution (CP‐2) at 4°C after recovery is presented. By these procedures, the mean recovery of red cells after lytic losses from freezing and thawing injury was 94.1%. The manipulations involved in transferring, mixing and resuspending the red cells removed an additional 11.1–16.2%, resulting in an overall recovery of 80.5% for serologic tests. Less than 3% of the cells exhibited cumulative lysis at seven days maintenance in CP‐2 solution at 4°C, and the blood group antigens examined remained as potent as those in sterile, fresh ACD blood.