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An Evaluation of the Single‐Channel Auto Analyzer in the Quantitation of Anti‐Rh 0
Author(s) -
Sturgeon Phillip,
Kaye Bea
Publication year - 1970
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1970.tb01491.x
Subject(s) - spectrum analyzer , calibration , limiting , calibration curve , chromatography , replication (statistics) , biomedical engineering , analytical chemistry (journal) , medicine , chemistry , detection limit , mathematics , statistics , optics , virology , physics , mechanical engineering , engineering
. Quantitation of Rh antibody with the Auto‐Analyzer is remarkably precise during short periods of replication. As the duration of operation extends over several hours considerable variation develops. This can be minimized with frequent calibrations. However, repeated intralaboratory tests on the same specimen over a period of several months show the range in values can vary from the mean by ±30%; with interlaboratory testing this may reach ±50%. Nevertheless, the AA technique does represent a distinct advance in the accuracy of Rh antibody quantitation when compared with established manual methods. With better control of the sources of variation such as drift in sensitivity and of cell base, and with extension of the accurate part of the calibration curve or limiting determinations to the most accurate portion, it should be possible to improve considerably the accuracy of the system. The correlations of prenatal serologic data determined quantitatively on the AA with severity of disease, were found, in these studies on a limited number of cases, not to be significantly better than those obtained with the semiquantitative manual methods employed. Studies of amniotic mud with enzyme treated cells gave the best prediction of severity of disease when done either by automated or manual methods.