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The Effect of Storage on Adhesion and Aggregation of Platelets 1
Author(s) -
Shively J. A.,
Gott Cora L.,
Jongh D. S.
Publication year - 1970
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1970.tb01450.x
Subject(s) - platelet , chemistry , adhesion , platelet aggregation , biophysics , in vitro , biochemistry , medicine , biology , organic chemistry
. Aggregation and adhesion of stored platelets were studied by a modified Wright technique. Comparisons of platelet rich plasma collected in ACD solution were made with and without aggregating agents, and at storage temperatures of 5°C, 24°C and 37°C. Platelets showed a loss of response to 0.5 μg ADP/ml, collagen and agar after storage for 4–6 h at the three temperatures with the greatest loss at 37°C and the least change at 5°C. The response to 5μ g ADP/ml also was best maintained at 5°C. After 6–8 h of storage at 5°C. there was some decrease in free (single) platelets after rotation in the absence of aggregating agents, with a marked decrease after 48 hours storage. With storage at 24°C and 37°C, decrease in single platelets after rotation was not demonstrated during the first 24 h. A response to 5 μg ADP/ml was maintained for more than a week with the 5°C preparations. Acidification diminished the response to ADP for the first few days of storage and also depressed platelet aggregation and adhesion in the absence of aggregating agents on prolonged storage. The results of this in vitro study would appear to correlate with the loss of hemostatic effect of stored platelets on transfusion. The sustained response to 5 μ g ADP/ml at 5°C suggests that this may be the best temperature for short term storage, although there is less spontaneous aggregation of platelets at the end of 24 h with 24°C storage. Also the ‘storage lesion’ appears to include loss of ADP or inability to release this substance from platelets.