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Action of Pronase and Neuraminidase on the Electrophoretic Mobility of Erythrocytes from Normal Cats and those with Spontaneous Tumours
Author(s) -
Mehrishi J. N.
Publication year - 1970
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1970.tb01426.x
Subject(s) - pronase , cats , neuraminidase , biology , electrophoresis , glycoprotein , biochemistry , glycolipid , chemistry , medicine , trypsin , enzyme
Summary. The electrophoretic mobility of cat erythrocytes has been determined and compared to the mobility of erythrocytes from some other animal species. The cellular mobility decreases by 53 to 70% on treatment with neuraminidase and increases with Pronase treatment of cells from normal cats by 18 to 36% and increases by about 18% in the case of cells from cats with spontaneous tumours (mammary and tongue carcinoma). This remarkable behaviour of Pronase‐treated erythrocytes has not been observed with Pronase‐treated erythrocytes from humans or other animal species and supports the model of the cat erythrocyte membrane with the extraordinary arrangement of glycoproteins and glycolipids proposed by Uhlenbruck.