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Hemolytic and Hemagglutinating Activities of 222 Plants
Author(s) -
Hossaini A. A.
Publication year - 1968
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1968.tb04083.x
Subject(s) - albumin , hemolysin , saline , hemagglutination , hemolysis , enzyme , chemistry , antigen , microbiology and biotechnology , immunology , biochemistry , biology , endocrinology , virulence , gene
Summary. The saline, the 30% albumin, and the enzyme Bromelin methods were used to detect the presence of hemagglutinins and hemolysins in the saline extracts of 222 plant seeds. Of these, 146 were non‐agglutinating, 56 agglutinating and 20 hemolytic. Of the 56 agglutinating lectins, one was of the specific type reacting with cells possessing blood group A 1 antigen only, and 55 were non‐specific. Of the latter group, 33 failed to agglutinate cells suspended in saline. Twenty‐eight of these required the addition of bromelin while five required the addition of either albumin or bromelin. Of the 20 hemolytic extracts, 19 showed hemolytic activity by all three methods of testing, while one hemolyzed red cells only when bromelin was added. Attempts to find lectins of anti‐U, anti‐k, and anti Lu b specificity failed. These results indicate that the enzyme technic is the most sensitive, followed by the albumin and saline methods.