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Antihemophilic Globulin (Factor VIII) Activity of Human Fibrinogen *
Author(s) -
Sgouris J. T.,
Hyndman L. A.,
Penner J. A.
Publication year - 1966
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1966.tb04212.x
Subject(s) - medicine , library science , health department , gerontology , family medicine , management , public health , pathology , computer science , economics
Antihemophilic globulin (AHG; Factor VIII) activity has been reported to deteriorate rapidly in blood stored at + 1°C to 6°C [4, 81. Because of this, Factor VIII-rich fibrinogen (Fraction I of COHN et al. [3]), is prepared from fresh blood and processing to the frozen state is completed within 24 h following donation. On the other hand, BRINKHOUS et al. [2] found 37% of the activity remaining after 21 days. BOWIE, THOMPSON and OWEN [l] found that one-half the normal level of Factor VIII activity was still present after 21 days of storage at 4°C. In this communication we show that clinically effective amounts of Factor VIII activity are also present in certain lots of dried fibrinogen (human) prepared from outdated citrated whole blood (21 days or older). Eighteen lots of human fibrinogen were assayed in vitro for their Factor VIII content by determining their ability to shorten the prolonged clotting time of recalcified hemophilic plasma, using a method by NILSSON, BLOMBACK and VON FRANCKEN [5]. The assay system consisted of 0.1 ml hemophilic plasma, 0.1 ml of a reference standard Factor VIII preparation (or fibrinogen in various dilutions) and 0.1 ml of 0.03 M CaCl,. The reference standard Factor VIII preparation was a lot of dried antihemophilic globulin (M.D.H. No.83). Fifty milligram (dry weight) aliquots of this reference material and of the test lots of dried fibrinogen were reconstituted in 5 ml saline This work was financed, in part, with funds and material provided under an agreement between the American National Red Cross and the Michigan Department of Health Laboratories.; the Michigan Heart Association and Public Health