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Cis ‐ and trans ‐acting genetic factors contribute to heterogeneity in the rate of crossing over between the D rosophila simulans clade species
Author(s) -
Cattani M. V.,
Kingan S. B.,
Presgraves D. C.
Publication year - 2012
Publication title -
journal of evolutionary biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.289
H-Index - 128
eISSN - 1420-9101
pISSN - 1010-061X
DOI - 10.1111/j.1420-9101.2012.02578.x
Subject(s) - biology , recombination , drosophila (subgenus) , genetics , genetic variation , drosophilidae , chromosome , evolutionary biology , drosophila melanogaster , gene
In the genus D rosophila , variation in recombination rates has been found within and between species. Genetic variation for both cis ‐ and trans ‐acting factors has been shown to affect recombination rates within species, but little is known about the genetic factors that affect differences between species. Here, we estimate rates of crossing over for seven segments that tile across the euchromatic length of the X chromosome in the genetic backgrounds of three closely related D rosophila species. We first generated a set of D rosophila mauritiana lines each having two semidominant visible markers on the X chromosome and then introgressed these doubly marked segments into the genetic backgrounds of its sibling species, D rosophila simulans and D rosophila sechellia . Using these 21 lines (seven segments, three genetic backgrounds), we tested whether recombination rates within the doubly marked intervals differed depending on genetic background. We find significant heterogeneity among intervals and among species backgrounds. Our results suggest that a combination of both cis ‐ and trans ‐acting factors have evolved among the three D . simulans clade species and interact to affect recombination rate.