The cost of mating rises nonlinearly with copulation frequency in a laboratory population of Drosophila melanogaster

Previous studies of Drosophila melanogaster have demonstrated a cost to females from male courtship and mating, but two critically important parameters remain unresolved: (i) the degree to which harm from multiple‐mating reduces lifetime fitness and (ii) how harm from mating might change with successive matings (rematings). Here we use ‘laboratory island analysis’ to quantify the costs that females incur with each remating, in the currency of lifetime fitness and under conditions that closely match those to which the flies have adapted for hundreds of generations. We experimentally manipulated the number of female matings by varying the order of daily 2‐h exposures of females to either sperm‐less males (XO) or intact males (XY). Females that mated more often had substantially reduced lifetime fecundity, and importantly, the fitness cost from remating rapidly accelerated.